切片時為準(zhǔn)確地切到組織，通常在包埋時組織會被輕輕 地壓在包埋模的底部。針吸穿刺活檢組織通常長10 ～ 20 mm，直徑卻＜ 1 mm，因此，即使有輕微的切削平面調(diào)節(jié)失誤， 也很容易導(dǎo)致活檢組織從一端被不均勻的切掉。當(dāng)切片的對象為一個幾乎透明的組織時，調(diào)節(jié)標(biāo)準(zhǔn)切削平面的問題可能會更復(fù)雜。另外，當(dāng)組織蠟塊分別在不同的切片機(jī)上切片 時，切削平面發(fā)生的變化會導(dǎo)致切片困難，比如，有些實(shí)驗(yàn)室在一臺切片機(jī)上修切蠟塊然后分發(fā)到其他切片機(jī)切片，或把蠟塊送到外院進(jìn)行會診需要重新切片。在這些情況下，的切削平面和隨后的切片機(jī)之間的誤差，往往由于再次修切必然會導(dǎo)致組織不必要的損失。
　　In order to cut the tissue accurately, the tissue will be pressed on the bottom of the embedding mold. Needle aspiration biopsy usually has a length of 10-20 mm and a diameter of less than 1 mm. Therefore, even if there is a slight adjustment error in the cutting plane, it is easy to cause the biopsy tissue to be cut off unevenly from one end. The problem of adjusting the standard cutting plane may be more complicated when the object of the slice is an almost transparent tissue. In addition, when tissue wax blocks are sliced on different slicers, the change of cutting plane will cause slicing difficulties. For example, some laboratories cut wax blocks on one slicer and then distribute them to other slicers for slicing, or send the wax blocks to the outside hospital for consultation, which needs to be re sliced. In these cases, the error between the first cutting plane and the subsequent slicer will inevitably lead to unnecessary tissue loss due to re cutting.

　　每次正式切片操作前，按照操作規(guī)范，制備標(biāo)準(zhǔn)的石蠟組織模塊，保證蠟塊與模型底部平面平行，無前后左右 傾斜的現(xiàn)象; 其次，利用空白標(biāo)準(zhǔn)蠟塊，分別將切片夾頭調(diào)節(jié) X 軸與刀刃接近平行，Y 軸與刀刃接近垂直; 如此得到的 切削平面我們稱之為該切片機(jī)的標(biāo)準(zhǔn)切削平面。它可以相 對固定，保持不動，進(jìn)行批量或連續(xù)處理切片操作時，可以在 一臺或多臺切片機(jī)上應(yīng)用。該切片方式不僅規(guī)范和簡化了 切片操作，避免因隨意調(diào)節(jié)切削平面帶來操作繁瑣和不 因素，更主要的是極大地提高了切片的效率和切片的質(zhì)量。
　　Before each formal slicing operation, firstly, prepare standard paraffin tissue module according to the operation specifications to ensure that the wax block is parallel to the bottom plane of the model, without the phenomenon of front and rear left and right inclination; secondly, use blank standard wax block to adjust the slicing chuck to the x-axis close to parallel to the blade, and the y-axis close to vertical to the blade; The cutting plane thus obtained is called the standard cutting plane of the slicer. It can be relatively fixed and kept still. It can be applied to one or more slicers when batch or continuous slicing operations are carried out. The slicing method not only standardizes and simplifies the slicing operation, avoids the tedious and unsafe factors caused by the arbitrary adjustment of cutting plane, but also greatly improves the efficiency and quality of slicing.
　　切片機(jī)切削平面的調(diào)節(jié)是通過 X 軸、Y 軸和鎖定旋鈕在內(nèi)的 3 個主要調(diào)節(jié)旋紐完成。操作時松弛鎖定旋鈕，當(dāng)?shù)玫綕M意的切削平面時，擰緊鎖定旋鈕，防止機(jī)械裝置產(chǎn)生進(jìn)一步的移動。對不同產(chǎn)地和型號的切片機(jī)來說，因安裝的標(biāo)本夾頭和調(diào)節(jié)系統(tǒng)類型不同，調(diào)節(jié)切削平面的精確步驟可有輕微差異，操作人員可依據(jù)切片機(jī)使用手冊做出調(diào)節(jié)。為了配合切削平面的順利調(diào)節(jié)，在包埋組織時還應(yīng)加強(qiáng)塑料包埋盒使用的操作規(guī)范，即待包埋模型底部石蠟開始凝固后再蓋上包埋盒，待其四周凝固后進(jìn)行二次加蠟。這樣的目的是使得包埋盒與組織蠟塊接觸更加穩(wěn)固，減少和防止切片時產(chǎn)生震顫或包埋盒與組織蠟塊脫落。另外，還需注意的是二次加蠟
　　The adjustment of the cutting plane of the slicer is completed by three main adjusting buttons, including the x-axis, Y-axis and locking knob. During operation, loosen the locking knob. When a satisfactory cutting plane is obtained, tighten the locking knob to prevent further movement of the mechanism. For slicers of different origins and models, the precise steps of adjusting the cutting plane can be slightly different due to the different types of sample collets and adjustment systems installed. The operator can make adjustment according to the slicer's operation manual. In order to coordinate with the smooth adjustment of the cutting plane, the operation specification for the use of the plastic embedding box should also be strengthened when embedding the structure, that is, after the paraffin at the bottom of the embedding model begins to solidify, the embedding box should be covered, and after the solidification around it, the wax should be added twice. The purpose of this method is to make the contact between the embedding box and the tissue wax block more stable, reduce and prevent the chattering or falling off of the embedding box and the tissue wax block when slicing. In addition, pay attention to the secondary waxing
　　時不能過滿，否則蠟塊不能與切片機(jī)夾具完全吻合，造成切片時松動震顫，影響切片質(zhì)量。
　　It should not be overfilled, otherwise the wax block can not be completely consistent with the fixture of the slicer, which will cause the loosening and shaking of the slicing and affect the quality of the slicing.
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